Molecular Identification and Characterization of Ph osphate Solubilizing Pseudomonas sp. Isolated from Rhizosphere of Mash Bean (Vigna Mungo L.) for Growth Promotion in Wheat

Abstract

Bio-inoculants have potential role in plant growth promotion. The present study evaluates the potential of Pseudomonas strains as bio-inoculants in wheat on the basis of plant growth promotion and physiological characterization. The 16S rRNA gene sequencing and phylogenetic analysis revealed that four isolated strains belonged to genus Pseudomonas. These strains were positive for phosphorus solubilization and indole acetic acid production, whereas only two strain were positive candidate for their nitrogen fixing ability as determined by presence or absence of nifH gene through amplification from polymerase chain reaction. The pot experiment showed that the integrated use of Pseudomonas strains as co-inoculant and 50% applied mineral fertilizers enhanced the maximum wheat growth and development from 58% to 140% for different shoot and root growth parameters. The strain NCCP-45 and NCCP-237 closely related to Pseudomonas beteli and Pseudomonas lini respectively. These isolated strains can be used to increase crop productivity by using as a bio-fertilizer inoculum.

Keywords: Bio-inoculant, PCR, PGPR, Phylogenetic analysis, 16S rRNA gene