Chrysin Modulates Aberrant Epigenetic Variations and Hampers the Migratory Behavior of Human Cervical Cells, HeLa
| dc.contributor.author | Raina, Ritu | |
| dc.contributor.author | Bagabir,Sali Abubaker | |
| dc.contributor.author | Almutary, Abdulmajeed | |
| dc.contributor.author | ETAL.. | |
| dc.date.accessioned | 2024-11-12T08:23:24Z | |
| dc.date.available | 2024-11-12T08:23:24Z | |
| dc.date.issued | 2022 | |
| dc.description | Regular cell functions are modifiable by different epigenetic modifications, and these alterations play a crucial role during cellular growth and development (Rahman et al., 2016; Shankar et al., 2016). The epigenetic modifications occur by the way of regulatory mechanisms involving histone modifications, DNA methylation, microRNAs, and chromatin remodeling that modulates gene expression and disturbs cellular machinery and homeostasis in cancer cells (Huang et al., 2011; Ong et al., 2011; You and Jones, 2012; Ho et al., 2013; Aggarwal et al., 2015; Busch et al., 2015; Shankar et al., 2016). DNA methylation at CpG residues of the promoters of tumor suppressor genes (TSGs) causes repression of tumor-suppressor genes (Hatzimichael and Crook, 2013; Shankar et al., 2016), which is considered as a key regulatory mechanism of gene silencing and is correlated with the overexpression of DNA methyltransferases (DNMTs) (Kogan et al., 2017; Li and Wang, 2017; Piyathilake et al., 2017). | |
| dc.description.abstract | Purpose: Plant-derived phytochemicals have shown epigenetic modulatory effect in different types of cancer by reversing the pattern of DNA methylation and chromatin modulation, thereby restoring the function of silenced tumor-suppressor genes. In the present study, attempts have been made to explore chrysin-mediated epigenetic alterations in HeLa cells. Methods: Colony formation and migration assays followed by methylation-specific PCR for examining the methylation status of CpG promoters of various tumor-suppressor genes (TSGs) and the expression of these TSGs at the transcript and protein levels were performed. Furthermore, global DNA methylation; biochemical activities of DNA methyltransferases (DNMTs), histone methyl transferases (HMTs), histone deacetylases (HDACs), and histone acetyl transferases (HATs) along with the expression analysis of chromatin-modifying enzymes; and H3 and H4 histone modification marks analyses were performed after chrysin treatment. Results: The experimental analyses revealed that chrysin treatment encourages cytostatic behavior as well as inhibits the migration capacity of HeLa cells in a time- and dose-dependent manner. Chrysin reduces the methylation of various tumor-suppressor genes, leading to their reactivation at mRNA and protein levels. The expression levels of various chromatin-modifying enzymes viz DNMTs, HMTs, HDACs, and HATS were found to be decreased, and H3 and H4 histone modification marks were modulated too. Also, reduced global DNA methylation was observed following the treatment of chrysin. Conclusion: This study concludes that chrysin can be used as a potential epigenetic modifier for cancer treatment and warrants for further experimental validation. | |
| dc.identifier.citation | Raina, R., Almutary, A. G., Bagabir, S. A., Afroze, N., Fagoonee, S., Haque, S., & Hussain, A. (2022). Chrysin modulates aberrant epigenetic variations and hampers migratory behavior of human cervical (HeLa) cells. Frontiers in Genetics, 12, 768130. | |
| dc.identifier.doi | https://doi.org/10.3389/fgene.2021.768130 | |
| dc.identifier.uri | https://repository.adu.ac.ae/handle/1/6993 | |
| dc.language.iso | en | |
| dc.publisher | Frontiers | |
| dc.title | Chrysin Modulates Aberrant Epigenetic Variations and Hampers the Migratory Behavior of Human Cervical Cells, HeLa | |
| dc.type | Article |