Bioactive potentials of endophyte (Fusarium redolens) isolated from Olea europaea

Abstract

During past few decades, endophytes have gained importance due their ability to produce bioactive compounds. Many medicinal plants are being exploited for the endophytic isolation to obtain drugs of interest. This study explored the fungal endophytes of Olive (Olea europaea L.) stem from which Fusarium redolens was selected for investigation of bioactive potential. The endophyte was identified using morphological characteristics and internal transcribed spacer ribosomal-deoxyribonucleic acid (ITS-rDNA) sequence analysis. The GCMS analysis of the crude extract yielded chrysophanol and fumaric acid. The culture filtrate of ethyl acetate extract showed significant cytotoxic potential against HepG2 cells, respectively. Furthermore, the screening of antioxidant potential of the ethyl acetate fungal extract using DPPH scavenging assay showed that Fusarium redolens extract exhibited potential activity with a significant EC50 value of 144.7 µg/mL.

Keywords: Bioactive compounds, Chrysophanol, Endophytic fungi, Fumaric acid, Fusarium redolens.