Gene tracking in a family of novel identical twins affected by severe type-III von Willebrand Disease (vWD)

Abstract

Informed consent was obtained from individuals before the start of the study. Blood sample was collected from the twins' patient and their family members in 3.2% sodium citrate (1:10), and centrifuge at 2500 rpm and 3500 rpm for 10 min. Platelet poor plasma (PPP) was frozen in aliquots at − 70 °C. Bleeding time was done by Ivy method [6]. The activated Partial Thromboplastin Time (aPTT) and prothrombin time (PT) tests were performed by automated coagulometer on twins patient's platelet poor. Studied twins and their family for different coagulation aspects showed that female twins were having severe type-III of vWD. Twins patient had no family history for such bleeding manifestation. Primary coagulation studies include bleeding time (BT), prothrombin time (PT), activated Partial Thromboplastin Time (aPTT) showed that both female twins were having prolonged BT and APTT (Table 1a) while other family members were normal for these primary coagulation screening tests.