The MTT and Crystal Violet Assays Potential Confounders in Nanoparticle Toxicity Testing
| dc.contributor.author | B.Sanderson | |
| dc.contributor.author | Almutary, Abdulmajeed | |
| dc.date.accessioned | 2024-10-31T09:20:11Z | |
| dc.date.available | 2024-10-31T09:20:11Z | |
| dc.date.issued | 2016-05-20 | |
| dc.description | The wide use of nanomaterials in many areas, including chem- istry, medicine, and biology, and incomplete characterization of the toxicity of many nanoparticles (NPs) heighten the demand for occupational health and safety guidelines.1,2 The creation of these guidelines relies on experimental investigation to charac- terize the framework for hazards and identification of the risk of exposure to newly synthesized NPs.3-6 Industrial workers are in daily risk from occupational exposure. 7-10 Therefore, it is essential to identify the cytotoxicity of these newly developed NPs.11-15 Research has identified the interference of some NPs with some assays, including Alamar Blue, neutral red (NR), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfo- phenyl)-2H-tetrazolium (WST-1).1 | |
| dc.description.abstract | The toxicological effects of nanoparticles (NPs) on humans, animals, and environment are largely unknown. Assessment of NPs cytotoxicity depends on the choice of the test system. Due to NPs optical activity and absorption values, they can influence the classical cytotoxicity assay. Eight NPs were spiked in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and crystal violet assays and tested with HaCaT human skin cells. The MTT assay standard curve optical density (OD) measurements were altered by the presence of trisilanol phenyl and trisilanol isooctyl polyhedral oligomeric silsesquioxane particles. The crystal violet standard curve OD measurements were significantly shifted by gold NPs, but they did not affect the MTT assay. Carbon black decreased ODs in the MTT and crystal violet assays and was localized in the cell cytoplasm. These findings strongly indicate that a careful choice of in vitro viability systems is required to avoid flawed measurement of NPs toxicity. Keywords: toxicity, nanoparticle, MTT assay, interference, crystal violet, trisilanol isooctyl, trisilanol cyclopentyl, trisilanol cyclohexyl, trisilanol phenyl, CdS QDs, silicon dioxide, AuNPs, carbon black, POSS particles | |
| dc.identifier.citation | Almutary, A., & Sanderson, B. J. S. (2016). The MTT and crystal violet assays: potential confounders in nanoparticle toxicity testing. International journal of toxicology, 35(4), 454-462. | |
| dc.identifier.doi | https://doi.org/10.1177/1091581816648906 | |
| dc.identifier.uri | https://repository.adu.ac.ae/handle/1/6912 | |
| dc.language.iso | en | |
| dc.publisher | SAGE Publications | |
| dc.title | The MTT and Crystal Violet Assays Potential Confounders in Nanoparticle Toxicity Testing | |
| dc.type | Article |